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bca protein assay kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bca protein assay kit
    Bca Protein Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+assay/BCA+Protein+Assay+Kit/10__22175_slash_mmb__17760-100-36-35
    Average 99 stars, based on 1 article reviews
    bca protein assay kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Protein Concentration:

    Article Title: Differential assembly of RNP granules via activation of distinct dsRNA sensors by adenovirus mutants
    Article Snippet: .. Protein concentration was determined using the Pierce 660 nm Protein Assay (Themo Scientific, 22660), and equal amounts of protein were reduced with 10 mM TCEP (Thermo Scientific, 77720), alkylated with 15 mM iodoacetamide (Sigma-Aldrich, I1149) in the dark at room temperature for 45 min and inactivated with 10 mM DTT in the dark at room temperature for 15 min. ..

    Article Title: Proximity Labeling Reveals RNA-Binding Proteins Associating with the Human Mitochondrial Import Receptor TOMM20
    Article Snippet: .. Protein concentration of the clarified lysates was determined with a Pierce 660 nm protein assay (Thermo, catalog no. 22660). ..

    Article Title: TNG961 is a selective oral HBS1L molecular glue degrader for the treatment of FOCAD-deleted cancers
    Article Snippet: .. Protein concentration was quantified using a BCA Protein Assay (Thermo Fisher Scientific 23227) or Pierce 660 nM Protein Assay (Thermo Fisher Scientific 22660). .. An equal volume of LDS sample buffer containing Dithiothreitol (DTT) (Thermo Fisher Scientific B0008/B0009) was added to the lysate and samples were heated at 95oC for 5–10 min. Proteins were resolved via electrophoresis on 4-12% Bis-Tris gels (Thermo Fisher Scientific WG1402BOX) (20-40 μg/lane) and transferred to nitrocellulose membranes (Thermo Fisher Scientific IB33002).

    Article Title: STAG2 loss amplifies EWS-FLI1-driven microsatellite enhancer activity promoting Ewing sarcoma aggressiveness.
    Article Snippet: .. Briefly, RIPA buffer (Thermo Fisher Scientific, 89900) with HaltTM Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific, 78440) was used to lyse the cells, followed by denaturation at 70 °C for 10 min, followed by measuring protein concentration using the Pierce 660 nm Protein Assay (Thermo Fisher Scientific, 22660). .. Between 10 to 30 μg of total protein per sample was resolved on either NuPAGE 3 to 8% Tris- Acetate or NuPAGE 4 to 12% Bis- Tris gels (Thermo Fisher Scientific), transferred to nitrocellulose membranes (Thermo Fisher Scientific, 88018) using a semidry transfer system (Trans- Blot Turbo; BioRad).

    Article Title: STAG2 loss amplifies EWS-FLI1-driven microsatellite enhancer activity promoting Ewing sarcoma aggressiveness
    Article Snippet: .. Briefly, RIPA buffer (Thermo Fisher Scientific, 89900) with HaltTM Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific, 78440) was used to lyse the cells, followed by denaturation at 70 °C for 10 min, followed by measuring protein concentration using the Pierce 660 nm Protein Assay (Thermo Fisher Scientific, 22660). .. Between 10 to 30 μg of total protein per sample was resolved on either NuPAGE 3 to 8% Tris-Acetate or NuPAGE 4 to 12% Bis-Tris gels (Thermo Fisher Scientific), transferred to nitrocellulose membranes (Thermo Fisher Scientific, 88018) using a semidry transfer system (Trans-Blot Turbo; Bio-Rad).

    Article Title: Chronopharmacological targeting of mitochondrial dihydroorotate dehydrogenase prevents diet-induced obesity in male mice
    Article Snippet: Homogenization of the samples was then performed mechanically using small metal balls and a tissue lyser (TissueLyser II, Qiagen). .. A portion of the recovered lysate was diluted 1:5 in UPLC ultrapure water before proceeding with a protein assay (Thermofisher, Pierce BCA Protein Assay Kit), to normalize the concentrations obtained by LC-MS/MS by the total protein concentration. .. To quantify uridine, dihydro-orotate (DHO) and orotate, 1 mM stock solutions of DHO (Sigma, D7003), orotate (Sigma, O2750) and uridine (Sigma, U3750) were prepared in UPLC-grade water and serially diluted to generate calibration standards from 0 to 100 μM in the corresponding matrix.

    Bicinchoninic Acid Protein Assay:

    Article Title: TNG961 is a selective oral HBS1L molecular glue degrader for the treatment of FOCAD-deleted cancers
    Article Snippet: .. Protein concentration was quantified using a BCA Protein Assay (Thermo Fisher Scientific 23227) or Pierce 660 nM Protein Assay (Thermo Fisher Scientific 22660). .. An equal volume of LDS sample buffer containing Dithiothreitol (DTT) (Thermo Fisher Scientific B0008/B0009) was added to the lysate and samples were heated at 95oC for 5–10 min. Proteins were resolved via electrophoresis on 4-12% Bis-Tris gels (Thermo Fisher Scientific WG1402BOX) (20-40 μg/lane) and transferred to nitrocellulose membranes (Thermo Fisher Scientific IB33002).

    Article Title: Chronopharmacological targeting of mitochondrial dihydroorotate dehydrogenase prevents diet-induced obesity in male mice
    Article Snippet: Homogenization of the samples was then performed mechanically using small metal balls and a tissue lyser (TissueLyser II, Qiagen). .. A portion of the recovered lysate was diluted 1:5 in UPLC ultrapure water before proceeding with a protein assay (Thermofisher, Pierce BCA Protein Assay Kit), to normalize the concentrations obtained by LC-MS/MS by the total protein concentration. .. To quantify uridine, dihydro-orotate (DHO) and orotate, 1 mM stock solutions of DHO (Sigma, D7003), orotate (Sigma, O2750) and uridine (Sigma, U3750) were prepared in UPLC-grade water and serially diluted to generate calibration standards from 0 to 100 μM in the corresponding matrix.

    Article Title: Metabolic regulation by tanycyte-derived extracellular vesicles through insulin precursor-mediated neuronal recognition and mTORC component delivery.
    Article Snippet: The hypothalamus regulates feeding and metabolic balance in response to metabolic cues.. Here we report that extracellular vesicles (EVs) are secreted from the mediobasal hypothalamus in a diurnal manner that is influenced by daily feeding.. Sox2-positive tanycytes have a critical role in maintaining the diurnal pattern of hypothalamic EV release.



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    MedChemExpress dynamin related protein 1 drp1 inhibitor mdivi 1
    USP18 aggravates cardiac I/R injury through regulation of mitochondria and inhibition of mitophagy. a Electron microscopy image showing mitophagy in USP18-cKO mouse hearts ( n= 5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. b Protein levels of PINK1, Parkin, ubiquitinated proteins (Ub), P62, and LC3II in mitochondria from heart tissue in USP18-cKO and WT mice 24 h after I/R injury ( n =4). c Electron microscopy image showing mitophagy in USP18-overexpres (OV) mouse hearts ( n =5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. d Protein levels of PINK1, Parkin, Ub, P62, and LC3II proteins in mitochondria from the heart tissue of USP18-OV mice 24 h after I/R injury ( n =4). Color shift in mitophagy dye (red) and lysosomal dye (green) in NRVMs showing mitophagy in NRVMs with USP18 siRNA transfection ( e ) or Ad-USP18 infection ( f ) and the quantitative mitophagy index in each group ( n= 5). Scale bar=9 μm. Protein levels of PINK1, Parkin, Ub, P62, and LC3II in mitochondria from NRVMs transfected with USP18 siRNA ( g ) or infected with Ad-USP18 ( h ). ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001 ⁎⁎⁎⁎ P <0.0001. USP18. Ubiquitin-specific protease 18; I/R. Ischemia/reperfusion; WT. Wild-type; KO. Knockout; P62. Sequestosome 1; LC3. Microtubule-associated <t>protein</t> <t>1</t> light chain 3; VDAC. Voltage-dependent anion channel.
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    Image Search Results


    USP18 aggravates cardiac I/R injury through regulation of mitochondria and inhibition of mitophagy. a Electron microscopy image showing mitophagy in USP18-cKO mouse hearts ( n= 5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. b Protein levels of PINK1, Parkin, ubiquitinated proteins (Ub), P62, and LC3II in mitochondria from heart tissue in USP18-cKO and WT mice 24 h after I/R injury ( n =4). c Electron microscopy image showing mitophagy in USP18-overexpres (OV) mouse hearts ( n =5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. d Protein levels of PINK1, Parkin, Ub, P62, and LC3II proteins in mitochondria from the heart tissue of USP18-OV mice 24 h after I/R injury ( n =4). Color shift in mitophagy dye (red) and lysosomal dye (green) in NRVMs showing mitophagy in NRVMs with USP18 siRNA transfection ( e ) or Ad-USP18 infection ( f ) and the quantitative mitophagy index in each group ( n= 5). Scale bar=9 μm. Protein levels of PINK1, Parkin, Ub, P62, and LC3II in mitochondria from NRVMs transfected with USP18 siRNA ( g ) or infected with Ad-USP18 ( h ). ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001 ⁎⁎⁎⁎ P <0.0001. USP18. Ubiquitin-specific protease 18; I/R. Ischemia/reperfusion; WT. Wild-type; KO. Knockout; P62. Sequestosome 1; LC3. Microtubule-associated protein 1 light chain 3; VDAC. Voltage-dependent anion channel.

    Journal: Military Medical Research

    Article Title: USP18 exacerbates myocardial I/R injury by inhibiting Parkin mitophagy through the deubiquitinase PTEN-L

    doi: 10.1016/j.mmr.2026.100004

    Figure Lengend Snippet: USP18 aggravates cardiac I/R injury through regulation of mitochondria and inhibition of mitophagy. a Electron microscopy image showing mitophagy in USP18-cKO mouse hearts ( n= 5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. b Protein levels of PINK1, Parkin, ubiquitinated proteins (Ub), P62, and LC3II in mitochondria from heart tissue in USP18-cKO and WT mice 24 h after I/R injury ( n =4). c Electron microscopy image showing mitophagy in USP18-overexpres (OV) mouse hearts ( n =5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. d Protein levels of PINK1, Parkin, Ub, P62, and LC3II proteins in mitochondria from the heart tissue of USP18-OV mice 24 h after I/R injury ( n =4). Color shift in mitophagy dye (red) and lysosomal dye (green) in NRVMs showing mitophagy in NRVMs with USP18 siRNA transfection ( e ) or Ad-USP18 infection ( f ) and the quantitative mitophagy index in each group ( n= 5). Scale bar=9 μm. Protein levels of PINK1, Parkin, Ub, P62, and LC3II in mitochondria from NRVMs transfected with USP18 siRNA ( g ) or infected with Ad-USP18 ( h ). ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001 ⁎⁎⁎⁎ P <0.0001. USP18. Ubiquitin-specific protease 18; I/R. Ischemia/reperfusion; WT. Wild-type; KO. Knockout; P62. Sequestosome 1; LC3. Microtubule-associated protein 1 light chain 3; VDAC. Voltage-dependent anion channel.

    Article Snippet: To block mitophagy, the selective dynamin-related protein 1 (Drp1) inhibitor Mdivi-1 was used (50 μmol/L, MedChemExpress, USA).

    Techniques: Inhibition, Electron Microscopy, Transfection, Infection, Ubiquitin Proteomics, Knock-Out